Fluctuación de la prevalencia viral en relación a Varroa destructor

Autores/as

  • Marcos Daniel Salina Laboratorio de Virología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata (UNLP)
  • Maricel Vega Curso de Producción Animal I
  • Maria Emilia Bravi Laboratorio de Virología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata (UNLP)
  • Barbara Bais Laboratorio de Virología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata (UNLP)
  • Hernan Sguazza Laboratorio de Virología, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata (UNLP)

DOI:

https://doi.org/10.52559/eunk.v1i2.25

Palabras clave:

Apis mellifera, co-infección, Varroa destructor, virus de las alas deformes

Resumen

Las abejas melíferas son de gran importancia para la humanidad, además de aportar una gran variedad de productos derivados de la colmena, polinizan ecosistemas y aumentan producciones agrícolas de importancia para el humano. Muchos patógenos pueden afectar la salud de las colonias y en ocasiones causar el colapso de las mismas, entre los principales agentes se destaca el acaro Varroa destructor. Además, este ectoparásito puede actuar como vector mecánico o biológico de diversos virus. En este trabajo 20 colmenas fueron muestreadas durante un año, se cuantificó porcentaje de infestación de Varroa destructor y se detectó la presencia de virus en muestras de abejas y en ácaros, con el objetivo de determinar la relación que poseen estos agentes durante las distintas estaciones del año. Demostramos que no hay asociación directa entre la presencia del ácaro y la mayoría de los virus estudiados, con excepción del virus de las alas deformes. En colmenas con mayor porcentaje de infestación por ácaros, se encontró con más frecuencia la presencia de este virus.

Citas

Aizen MA & Harder LD. The global stock of domesticated honey bees is growing slower than agricultural demand for pollination. Curr. Biol. 2009; 19: 915-918. DOI: https://doi.org/10.1016/j.cub.2009.03.071

Allen M, Ball BV. The incidence and world distribution of honey bee viruses. J Bee World.1996; 77(3):141–162. https://doi. org/10.1080/0005772X.1996.11099306 DOI: https://doi.org/10.1080/0005772X.1996.11099306

Annoscia D, Brown SP, Di Prisco G, De Paoli E, Del Fabbro S, Frizzera D, Zanni V, Galbraith DA, Caprio E, Grozinger CM, Pennacchio F, Nazzi F. Haemolymph removal by Varroa mite destabilizes the dynamical interaction between immune efectors and virus in bees, as predicted by Volterra’s model. Proc R Soc B. 2019; 286:20190331. https://doi.org/10.1098/rspb.2019.0331 DOI: https://doi.org/10.1098/rspb.2019.0331

Bailey L, Ball BV, Perry JN. Association of viruses with two protozoal pathogens of the honey bee. Ann Appl Biol. 1983; 103(1):13–20. https://doi.org/10.1111/j.1744-7348.1983.tb027 35.x DOI: https://doi.org/10.1111/j.1744-7348.1983.tb02735.x

Bailey L, Carpenter JM, Woods RD. Egypt bee virus and Australian isolates of Kashmir bee virus. J Gen Virol. 1979;43:641–7 DOI: https://doi.org/10.1099/0022-1317-43-3-641

Bailey L. The incidence of virus disease in the honeybee. Ann. Appl. Biol. 1967, 60: 43-48. DOI: https://doi.org/10.1111/j.1744-7348.1967.tb05920.x

Ball, B.V. & Allen, M.F. The prevalence of pathogens in the honey bee (Apis mellifera) colonies infested with the parasitic mite Varroa jacobsoni. Ann. Appl. Biol. 1988; 113: 237-244. DOI: https://doi.org/10.1111/j.1744-7348.1988.tb03300.x

Beaurepaire, A.; Piot, N.; Doublet, V.; Antunez, K.; Campbell, E.; Chantawannakul, P.; Chejanovsky, N.; Gajda, A.; Heerman, M.; Panziera, D.; et al. Diversity and Global Distribution of Viruses of the Western Honey Bee, Apis mellifera. Insects 2020; 11, 239. DOI: https://doi.org/10.3390/insects11040239

Bodden, JM; A Jenny; E Hazlehurst; M Erin & R Wilson. Floral traits predict frequency of defecation on flowers by foraging bumble bees. Journal of Insect Science. 2019; 19(5):2. DOI: https://doi.org/10.1093/jisesa/iez091

Brutscher, L.M. Daughenbaugh K, Flenniken LM. Antiviral defense mechanisms in honey bees. Current Opinion in Insect Science. 2015; 10; 71-82. DOI: https://doi.org/10.1016/j.cois.2015.04.016

Brutscher, L.M., McMenamin AJ, Flenniken ML. The Buzz about Honey Bee Viruses. PLoS Pathogens. 2016; 12(8), e1005757, https://doi.org/10.1371/journal.ppat.1005757. DOI: https://doi.org/10.1371/journal.ppat.1005757

Cornman RS. Relative abundance and molecular evolution of Lake Sinai Virus (Sinaivirus) clades. PeerJ. 2019; 7:e6305 DOI 10.7717/peerj.6305 DOI: https://doi.org/10.7717/peerj.6305

Cresswell JE, Desneux N, Vanengelsdorp D. Dietary traces of neonicotinoid pesticides as a cause of population declines in honey bees: an evaluation by Hill’s epidemiological criteria. Pest Manag Sci.2012; 68: 819–827. DOI: https://doi.org/10.1002/ps.3290

Dalmon A, Diévart V, Thomasson M, Fouque R, Vaissière BE, Guilbaud L, Le Conte Y, Henry M. Possible Spillover of Pathogens between Bee Communities Foraging on the Same Floral Resource. Insects. 2021; 12, 122 DOI: https://doi.org/10.3390/insects12020122

de Miranda JR, Cordoni G, Budge G. The Acute bee paralysis virus–Kashmir bee virus–Israeli acute paralysis virus complex. J Invertebr Pathol. 2010; 103(1):30–47. https ://doi.org/10.1016/j.jip.2009.06.014 DOI: https://doi.org/10.1016/j.jip.2009.06.014

Decourtye, A., Mader, E., and Desneux, N. Landscape enhancement of floral resources for honey bees in Agro-ecosystems. Apidologie. 2010; 41: 264–277 DOI: https://doi.org/10.1051/apido/2010024

Di Prisco G, Annoscia D, Margiotta M, Ferrara R, Varricchio P, Zanni V, Caprio E, Nazzi F, Pennacchio F. A mutualistic symbiosis between a parasitic mite and a pathogenic virus undermines honey bee immunity and health. Proc Natl Acad Sci. 2016; 113:3203–3208. https://doi.org/10.1073/pnas.1523515113 DOI: https://doi.org/10.1073/pnas.1523515113

Dietemann, V., Nazzi, F., Martin, S.J., Anderson, D., Locke, B. et al. Standard methods for Varroa research. In V Dietemann; J D Ellis; P Neusmann (Eds), The COLOSS BEEBOOK, Volume II: standard methods for Apis mellifera pest and pathogen research. J. Apic. Res.2013; 52(4). doi: 10.3896/IBRA.1.52.1.09 DOI: https://doi.org/10.3896/IBRA.1.52.4.16

Fernández de Landa G, Brasesco C, Quintana S, Di Gerónimo V, Junges C, Porrini L, Reynaldi JF, Eguaras M, Maggi M. Lake sinai virus (LSV), un novedoso virus que se encuentra infectando las abejas melíferas en Argentina. Centro de Investigación En Abejas Sociales—CIAS.2020; 36:14–16.

Francis, R M; Nielsen, S L; Kryger, P. Patterns of viral infection in honey bee queens. Journal of General Virology. 2013; 94(3): 668-676. http://dx.doi.org/10.1099/vir.0.047019-0 DOI: https://doi.org/10.1099/vir.0.047019-0

Gauthier L, Cornman S, Hartmann U, Cousserans F, Evans JD, de Miranda JR, Neumann P. The Apis mellifera Filamentous Virus Genome. Viruses. 2015; 7, 3798-3815; doi:10.3390/v7072798 DOI: https://doi.org/10.3390/v7072798

Giacobino, A, Molineri AI, Pacini A, Fondevila N, Pietronave H, Rodr_ıguez G, Palacio A, Bulacio Cagnolo N, Orellano E, Salto CE. Signorini ML, Merke J. Varroa destructor and viruses association in honey bee colonies under different climatic conditions. Environmental Microbiology Reports. 2016; doi:10.1111/1758-2229.12410 DOI: https://doi.org/10.1111/1462-2920.13297

ICTV International Committee on Taxonomy of Viruses (2020) https://talk.ictvonline.org/taxonomy/ Accessed May 2021.

Martin S. A population model for the ectoparasitic mite Varroa jacobsoni in honey bee (Apis mellifera) colonies. Ecological Modelling. 1998; 109 267–281 DOI: https://doi.org/10.1016/S0304-3800(98)00059-3

McMenamin AJ, Genersch E. Honey bee colony losses and associated viruses. Curr Opin Insect Sci. 2015; 8:121-129. DOI: https://doi.org/10.1016/j.cois.2015.01.015

Meana A, Llorens-Picher M, Euba A, Bernal JL, Bernal J, Garcia-Chao M, Dagnac T, Castro-Hermida JA, Gonzalez-Porto AV, Higes M, Martin-Hernández R. Risk factors associated with honey bee colony loss in apiaries in Galicia, NW Spain. Span. J Agric Res. 2017; 15 (1): e0501. DOI: https://doi.org/10.5424/sjar/2017151-9652

Molineri A, Giacobino A, Pacini A, Bulacio Cagnolo N, Fondevila N, Ferrufino C, Merke J, Orellano E, Bertozzi E, Masciángelo G, Pietronave H, Signorini M. Risk factors for the presence of Deformed wing virus and Acute bee paralysis virus under temperate and subtropical climate in Argentinian bee colonies. Prev Vet Med. 2017; 1;140:106-115. doi: 10.1016/j.prevetmed.2017.02.019. b DOI: https://doi.org/10.1016/j.prevetmed.2017.02.019

Molineri, A., Pacini A, Giacobino A, Bulacio-Cagnolo N, Aignasse A, Zago 4, Fondevila LN, Ferrufino C, Merke J, Orellano E, Bertozzi E, Pietronave H, Signorini ML. Prevalence of honey bee (Apis mellifera) viruses in temperate and subtropical regions from Argentina. Rev Argent Microbiol. 2017; 49(2):166-173. doi: 10.1016/j.ram.2016.12.004. a DOI: https://doi.org/10.1016/j.ram.2016.12.004

Mondet, F., de Miranda, J.R., Kretzschmar, A., Le Conte, Y., and Mercer, A.R. On the front line: quantitative virus dynamics in honeybee (Apis mellifera L.) colonies along a new expansion front of the parasite Varroa destructor. PLoS Pathog. 2014; 10 (8): e1004323. doi:10.1371/ journal.ppat.1004323 DOI: https://doi.org/10.1371/journal.ppat.1004323

Mordecai GJ, Wilfert L, Martin SJ, Jones IM, Schroeder DC. Diversity in a honey bee pathogen: First report of a third master variant of the Deformed wing wirus quasispecies. ISME J. 2016;10:1264–73. DOI: https://doi.org/10.1038/ismej.2015.178

Ongus JR (2006) Varroa destructor virus 1: a new picorna-like virus in Varroa mites as well as honey bees. Thesis Wageningen University—with references—with summary in Dutch ISBN 90-8504-363-8

Posada-Florez F, Childers AK, Heerman MC, Egekwu NI, Cook SC, Chen Y, Evans JD, Ryabov E. Deformed wing virus type A, a major honey bee pathogen, is vectored by the mite Varroa destructor in a non-propagative manner. Sci Rep. 2019; 9:12445. https://doi.org/10.1038/s41598-019-47447-3 DOI: https://doi.org/10.1038/s41598-019-47447-3

Quintana S, Brasesco C, Porrini LP, Di Gerónimo V, Eguaras MJ, Maggi M. First molecular detection of Apis mellifera flamentous virus (AmFV) in honey bees (Apis mellifera) in Argentina. J Apic Res. 2019; 59(2):211–217. https://doi.org/10.1080/00218 839.2019.1690100

Ramsey SD, Ronal O, Bauchan G, Gulbronson C, Mowery JD, Cohen A, Lim D, Joklik J, Cicero JM, Ellis JD, Hawthorne D, vanEngelsdorp D. Varroa destructor feeds primarily on honey bee fat body tissue and not hemolymph. Proc Natl Acad Sci. 2019; 116(5):1792–1801. https://doi.org/10.1073/pnas.1818371116 DOI: https://doi.org/10.1073/pnas.1818371116

Remnant, E.J., Shi, M., Buchmann, G., Blacquière, T., Holmes, E.C., Beekman, M., Ashed, A. A diverse range of novel RNA viruses in geographically distinct honey bee populations. Journal of Virology. 2017; v.91, n.16, p. e00158-17. DOI: https://doi.org/10.1128/JVI.00158-17

Retschnig G, Kellermann LA, Mehmann MM, Yañez O, Winiger P, Williams GR, Neumann P. Black queen cell virus and drifting of honey bee workers (Apis mellifera). J Apic Res. 2019; 58(5):754–755. https://doi.org/10.1080/00218839.2019.1655133 DOI: https://doi.org/10.1080/00218839.2019.1655133

Reynaldi JF, Sguazza GH, Pecoraro MR, Tizzano MA, Galosi CM. First report of viral infections that afect Argentine honeybees. Environ Microbiol Rep. 2010; 2(6):749–751. https://doi.org /10.1111/j.1758-2229.2010.00173.x DOI: https://doi.org/10.1111/j.1758-2229.2010.00173.x

Reynaldi JF, Sguazza GH, Tizzano MA, Fuentealba N, Galosi CM, Pecoraro MR. First report of Israeli acute paralysis virus in asymptomatic hives of Argentina. Revista Argentina de Microbiología. 2011; 43(2):84–86. https://doi.org/10.1590/S0325 -75412011000200003

Riveros G, Arismendi N, Zapata N, Smagghe G, Rodríguez M, Gerding M, Vargas M. A scientific note on first detection of Kashmir bee virus in Apis mellifera (Hymenoptera: Apidae) in South America. Apidologie. 2018; 49(2):220–223. https ://doi.org/10.1007/s1359 2-017-0545-z DOI: https://doi.org/10.1007/s13592-017-0545-z

Salina MD, Genchi Garcia ML, Bais B, Bravi ME, Brasesco C, Maggi M, Pecoraro M, Larsen A, Sguazza GH, Reynaldi FJ. Viruses that afect Argentinian honey bees (Apis mellifera) Archives of Virology. 2021; https://doi.org/10.1007/s00705-021-05000-6 DOI: https://doi.org/10.1007/s00705-021-05000-6

Sguazza GH, Reynaldi FJ, Galosi CM, Pecoraro RM. Simultaneous detection of bee viruses by multiplex PCR. J Virol Methods. 2013; 194(1–2):102–106. https://doi.org/10.1016/j.jviro met.2013.08.003 DOI: https://doi.org/10.1016/j.jviromet.2013.08.003

Shen MQ, Cui LW, Ostiguy N, Cox-Foster D. Intricate transmission routes and interactions between picorna-like viruses (Kashmir bee virus and sacbrood virus) with the honeybee host and the parasitic varroa mite. Journal of General Virology. 2005; 86: 2281–2289 DOI: https://doi.org/10.1099/vir.0.80824-0

Susevich ML, Genchi Garcia ML, Bravi ME, Echeverria MG, Reynaldi FJ. RNA virus circulation among pollinator and non-pollinator species in Argentina. Ecología Austral. 2021; 31:413-419. https://doi.org/10.25260/EA.21.31.3.0.1508 DOI: https://doi.org/10.25260/EA.21.31.3.0.1508

vanEngelsdorp D, Traynor KS, Andree M, Lichtenberg EM, Chen Y, Saegerman C, Cox-Foster DL. Colony Collapse Disorder (CCD) and bee age impact honey bee pathophysiology PLOS ONE. 2017; https://doi.org/10.1371/journal.pone.0179535 DOI: https://doi.org/10.1371/journal.pone.0179535

Yañez O, Piot N, Dalmon A, de Miranda JR, Chantawannakul P, Panziera D, Amiri E, Smagghe G, Schroeder D, Chejanovsky N. Bee Viruses: Routes of Infection in Hymenoptera. Front. Microbiol. 2020; doi: 10.3389/fmicb.2020.00943 DOI: https://doi.org/10.3389/fmicb.2020.00943

Yue C, Genersch, E. RT-PCR analysis of Deformed wing virus (DWV) in bees (Apis mellifera) and mites (Varroa destructor). J. Gen. Virol.2015; 86, 3419–3424. DOI: https://doi.org/10.1099/vir.0.81401-0

Zhang, Yakun, Meiling Su, Long Wang, Shaokang Huang, Songkun Su, and Wei-Fone Huang. "Vairimorpha (Nosema) ceranae Infection Alters Honey Bee Microbiota Composition and Sustains the Survival of Adult Honey Bees" Biology. 2021; 10, no. 9: 905. https://doi.org/10.3390/biology10090905 DOI: https://doi.org/10.3390/biology10090905

Descargas

Publicado

2023-01-11

Cómo citar

Salina, M. D., Vega, M., Bravi, M. E., Bais, B., & Sguazza, H. (2023). Fluctuación de la prevalencia viral en relación a Varroa destructor. Eunk, 1(2), 3–11. https://doi.org/10.52559/eunk.v1i2.25

Número

Sección

Artículos Originales

Categorías

Datos de los fondos